The early expansion and evolutionary dynamics of POU class genes.

The POU genes represent a diverse class of animal-specific transcription factors that play important roles in neurogenesis, pluripotency, and cell-type specification. Although previous attempts have been made to reconstruct the evolution of the POU class, these studies have been limited by a small number of representative taxa, and a lack of sequences from basally branching organisms. In this study, we performed comparative analyses on available genomes and sequences recovered through "gene fishing" to better resolve the topology of the POU gene tree. We then used ancestral state reconstruction to map the most likely changes in amino acid evolution for the conserved domains. Our work suggests that four of the six POU families evolved before the last common ancestor of living animals-doubling previous estimates-and were followed by extensive clade-specific gene loss. Amino acid changes are distributed unequally across the gene tree, consistent with a neofunctionalization model of protein evolution. We consider our results in the context of early animal evolution, and the role of POU5 genes in maintaining stem cell pluripotency

Attempted DNA extraction from a Rancho La Brea Columbian mammoth (Mammuthus columbi): prospects for ancient DNA from asphalt deposits.

Fossil-bearing asphalt deposits are an understudied and potentially significant source of ancient DNA. Previous attempts to extract DNA from skeletons preserved at the Rancho La Brea tar pits in Los Angeles, California, have proven unsuccessful, but it is unclear whether this is due to a lack of endogenous DNA, or if the problem is caused by asphalt-mediated inhibition. In an attempt to test these hypotheses, a recently recovered Columbian mammoth (Mammuthus columbi) skeleton with an unusual pattern of asphalt impregnation was studied. Ultimately, none of the bone samples tested successfully amplified M. columbi DNA. Our work suggests that reagents typically used to remove asphalt from ancient samples also inhibit DNA extraction. Ultimately, we conclude that the probability of recovering ancient DNA from fossils in asphalt deposits is strongly (perhaps fatally) hindered by the organic compounds that permeate the bones and that at the Rancho La Brea tar pits, environmental conditions might not have been ideal for the general preservation of genetic material

Food addiction and psychosocial adversity: Biological embedding, contextual factors, and public health implications

The role of stress, trauma, and adversity particularly early in life has been identified as a contributing factor in both drug and food addictions. While links between traumatic stress and substance use disorders are well documented, the pathways to food addiction and obesity are less established. This review focuses on psychosocial and neurobiological factors that may increase risk for addiction-like behaviors and ultimately increase BMI over the lifespan. Early childhood and adolescent adversity can induce long-lasting alterations in the glucocorticoid and dopamine systems that lead to increased addiction vulnerability later in life. Allostatic load, the hypothalamic-pituitary-adrenal axis, and emerging data on epigenetics in the context of biological embedding are highlighted. A conceptual model for food addiction is proposed, which integrates data on the biological embedding of adversity as well as upstream psychological, social, and environmental factors. Dietary restraint as a feature of disordered eating is discussed as an important contextual factor related to food addiction. Discussion of various public health and policy considerations are based on the concept that improved knowledge of biopsychosocial mechanisms contributing to food addiction may decrease stigma associated with obesity and disordered eating behavior

Poética del Postismo en la obra de Eduardo Chicharro

Este artículo estudia los métodos característicos de la poesía del "Postismo" en la obra de Eduardo Chicharro, teniendo en cuenta la base teórica que define la estética de esta corriente literaria. Los rasgos estilísticos en trabajos de este escritor y de esta tendencia vanguardista de posguerra se caracterizan por el uso de dispositivos musicales, semánticas y sintácticos, que se analizan en el curso de este trabajo.Este artículo estudia los métodos característicos de la poesía del "Postismo" en la obra de Eduardo Chicharro, teniendo en cuenta la base teórica que define la estética de esta corriente literaria. Los rasgos estilísticos en trabajos de este escritor y de esta tendencia vanguardista de posguerra se caracterizan por el uso de dispositivos musicales, semánticas y sintácticos, que se analizan en el curso de este trabajo.notPeerReviewe

Effects of emotional salience and semantic domain on cross-form priming

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal

Backreaction of Schwinger pair creation in massive QED2_2

Particle-antiparticle pairs can be produced by background electric fields via the Schwinger mechanism provided they are unconfined. If, as in QED in (3+1)-$d$ these particles are massive, the particle production rate is exponentially suppressed below a threshold field strength. Above this threshold, the energy for pair creation must come from the electric field itself which ought to eventually relax to the threshold strength. Calculating this relaxation in a self-consistent manner, however, is difficult. Chu and Vachaspati addressed this problem in the context of capacitor discharge in massless QED$_2$ [1] by utilizing bosonization in two-dimensions. When the bare fermions are massless, the dual bosonized theory is free and capacitor discharge can be analyzed exactly [1], however, special care is required in its interpretation given that the theory exhibits confinement. In this paper we reinterpret the findings of [1], where the capacitors Schwinger-discharge via electrically neutral dipolar meson-production, and generalize this to the case where the fermions have bare masses. Crucially, we note that when the initial charge of the capacitor is large compared to the charge of the fermions, $Q \gg e$, the classical equation of motion for the bosonized model accurately characterizes the dynamics of discharge. For massless QED$_2$, we find that the discharge is suppressed below a critical plate separation that is commensurate with the length scale associated with the meson dipole moment. For massive QED$_2$, we find in addition, a mass threshold familiar from (3+1)-$d$, and show the electric field relaxes to a final steady state with a magnitude proportional to the initial charge. We discuss the wider implications of our findings and identify challenges in extending this treatment to higher dimensions.Comment: 21 pages, 2 figures; several additional references and enhanced discussion. Matches JHEP versio

Paleoproterozoic sterol biosynthesis and the rise of oxygen

Natural products preserved in the geological record can function as ‘molecular fossils’, providing insight into organisms and physiologies that existed in the deep past. One important group of molecular fossils is the steroidal hydrocarbons (steranes), which are the diagenetic remains of sterol lipids. Complex sterols with modified side chains are unique to eukaryotes, although simpler sterols can also be synthesized by a few bacteria. Sterol biosynthesis is an oxygen-intensive process; thus, the presence of complex steranes in ancient rocks not only signals the presence of eukaryotes, but also aerobic metabolic processes. In 1999, steranes were reported in 2.7 billion year (Gyr)-old rocks from the Pilbara Craton in Australia, suggesting a long delay between photosynthetic oxygen production and its accumulation in the atmosphere (also known as the Great Oxidation Event) 2.45–2.32 Gyr ago. However, the recent reappraisal and rejection of these steranes as contaminants pushes the oldest reported steranes forward to around 1.64 Gyr ago (ref. 6). Here we use a molecular clock approach to improve constraints on the evolution of sterol biosynthesis. We infer that stem eukaryotes shared functionally modern sterol biosynthesis genes with bacteria via horizontal gene transfer. Comparing multiple molecular clock analyses, we find that the maximum marginal probability for the divergence time of bacterial and eukaryal sterol biosynthesis genes is around 2.31 Gyr ago, concurrent with the most recent geochemical evidence for the Great Oxidation Event. Our results therefore indicate that simple sterol biosynthesis existed well before the diversification of living eukaryotes, substantially predating the oldest detected sterane biomarkers (approximately 1.64 Gyr ago), and furthermore, that the evolutionary history of sterol biosynthesis is tied to the first widespread availability of molecular oxygen in the ocean–atmosphere system

Developments in Business Gaming A Review of the Past 40 Years

This article examines developments in business simulation gaming during the past 40 years. Covered in this article are a brief history of business games, the changing technology employed in the development and use of business games, changes in why business games are adopted and used, changes in how business games are administered, and the current state of business gaming. Readers interested in developments in other areas of simulation gaming (urban planning, social studies, ecology, economics, geography, health, etc.) are encouraged to look at other articles appearing during the 40th anniversary year of Simulation & Gaming and at the many fine articles that appeared in the silver anniversary issue of Simulation & Gaming (December 1995)

Cardiorespiratory Fitness Diminishes the Effects of Age on White Matter Hyperintensity Volume

White matter hyperintensities (WMHs) are among the most commonly observed marker of cerebrovascular disease. Age is a key risk factor for WMH development. Cardiorespiratory fitness (CRF) is associated with increased vessel compliance, but it remains unknown if high CRF affects WMH volume. This study explored the effects of CRF on WMH volume in community-dwelling older adults. We further tested the possibility of an interaction between CRF and age on WMH volume. Participants were 76 adults between the ages of 59 and 77 (mean age = 65.36 years, SD = 3.92) who underwent a maximal graded exercise test and structural brain imaging. Results indicated that age was a predictor of WMH volume (beta = .32, p = .015). However, an age-by-CRF interaction was observed such that higher CRF was associated with lower WMH volume in older participants (beta = -.25, p = .040). Our findings suggest that higher levels of aerobic fitness may protect cerebrovascular health in older adults